古典H1N1亚型猪流感病毒PB2 K627E突变对病毒复制能力的影响

doi:10.11843/j.issn.0366-6964.2016.03.017

Abstract

Abstract:

This experiment was conducted to explore the effect of PB2 K627E mutation on the replication competence of classical H1N1 subtype swine influenza virus(A/Swine/Guangdong/1/2011).The rescued and mutant strains of classical H1N1 subtype swine influenza virus were obtained by reverse genetics and point mutation technology，and researches were taken on MDCK cells and mice.The results on MDCK cells showed that，compared with the rescued strain，the mutant virus had a slight degree of CPE；the morphology of plaque of mutant virus was much smaller；the growth curve showed that it had a significant distinct difference.The results of experiments in mice showed that the mutant strain had a low pathogenicity and could not lead the mice loss weight，and virus titer in lung tissues on the 3rd and 5th day post challenge showed that there was a significant distinct difference between the two strains and the pathological damage caused by mutant one was not serious.The results on MDCK and mice both showed that the mutant strain’s replication competence was lower than the rescued one.This conclusion demonstrates that the amino acid of PB2 627 is not only one of the important virulence markers，but also provided the condition to further clarify its pathogenic mechanism.

CLC Number:

S852.659.5

WANG Xiu-hui，GONG Xiao-qian，RUAN Bao-yang，LIU Xiao-min，WANG Qi，ZHANG Peng，LI Ze-jun，ZHOU Yan-jun，TONG Wu，ZHENG Hao，TONG Guang-zhi，YU Hai. The Effect of PB2 K627E Mutation on the Replication Competence of Classical H1N1 Subtype Swine Influenza Virus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(3): 543-548.

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The Effect of PB2 K627E Mutation on the Replication Competence of Classical H1N1 Subtype Swine Influenza Virus

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